口腔疾病防治 ›› 2017, Vol. 25 ›› Issue (8): 498–500.doi: 10.12016/j.issn.2096-1456.2017.08.005

• 基础研究 • 上一篇    下一篇

Beagle 犬下颌重度牙槽嵴萎缩动物模型的建立

吕影涛1(), 苏媛2, 马伟群3, 刘淑英3, 徐平平3   

  1. 1. 南方医科大学口腔医院海珠广场院区种植修复科,广东广州(510280)
    2. 南方医科大学口腔医院牙周种植科,广东广州(510280)
    3. 南方医科大学口腔医院口腔颌面外科,广东广州(510280)
  • 收稿日期:2016-12-31 修回日期:2017-04-07 出版日期:2017-08-20 发布日期:2018-09-03
  • 作者简介:

    作者简介:吕影涛,主治医师,硕士, Email:0370lvyingtao@163.com

  • 基金资助:
    国家自然科学基金(81271187);广东省医学科学技术研究基金(A2015595)

Construction of the animal model of mandibular severe alveolar bone atrophy in Beagle dogs

Yingtao LV1(), Yuan SU2, Weiqun MA3, Shuying LIU3, Pingping XU3   

  1. 1. Department of Oral Implantology Stomatological Hospital Southern Medical University Guangzhou 510280 China
    2. Department of Implantrestoration Stomatological Hospital Southern Medical University Guangzhou 510280 China
    3. Department of Oral and Maxillofacial surgery Stomatological Hospital Southern Medical University Guangzhou 510280 China
  • Received:2016-12-31 Revised:2017-04-07 Online:2017-08-20 Published:2018-09-03

摘要:

目的 建立一种与临床相符的下颌重度牙槽嵴萎缩的动物模型。方法 以Beagle犬为实验动物,拔除其双侧下颌第四前磨牙和第一磨牙,在距下颌第三前磨牙及下颌第二磨牙釉牙骨质界8 mm处的牙槽嵴上水平磨一凹线,咬骨钳去除该线上方的牙槽骨,用骨凿将去骨区域修平,形成25 mm × 8 mm大小的箱状缺损洞形,置入无菌硅胶假体,严密缝合后,待其自然愈合。术后8周行锥形束CT检查。结果 骨改建8周后术区牙槽嵴顶呈圆弧形,嵴顶最低点至下牙槽神经管的距离平均2.5 mm。结论 本研究成功构建了Beagle犬下颌重度牙槽嵴萎缩动物模型,为进行垂直骨增量相关实验奠定了实验基础。

关键词: 牙槽嵴萎缩, 动物模型, 垂直骨增量

Abstract:

Objective To establish an animal model that clinically conforms to the characteristics of severe alveolar ridge atrophy. Methods Beagle dogs were used as experimental subjects. Bilateral fourth premolars and first molars in mandible were extracted. A horizontal groove was made on alveolar ridge which was 8 mm from the cemento-enamal junction of mandible third premolar and second molar. Rongeur was used to remove the alveolar bone above this groove and bone chisel was used to level the bone-free area. A box-shaped defect cavity of the size 25 mm × 8 mm was formed with sterile silicone prosthesis implanted. After careful suture we waited for the subjects to heal naturally. Eight weeks after operation, CBCT examination was performed. Results Eight weeks after bone remodeling the top of alveolar ridge of operation area appeared to be a circular arc. The average distance from the bottom of the ridge to inferior alveolar nerve canal was 2.5 mm. Conclusion This study successfully established the Beagle dog animal model for severe alveolar ridge atrophy and laid the foundation for experiments on vertical bone augmentation.

Key words: Alveolar bone atrophy, Animal model, Vertical bone augmentation

中图分类号: 

  • R788

图1

拔牙及骨缺损制备"

图2

骨改建8周后CBCT检查及测量"

[1] Moy PK, Medina D, Shetty V, et al.Dental implant failure rates and associated risk factors[J]. Int J Oral Maxllofac Implants, 2005, 20(4): 569-577.
[2] Araújo MG, Lindhe J.Dimensional ridge alterations following tooth extraction. An experimental study in the dog[J]. J Clin Periodontol, 2005, 32(2): 212-218.
[3] Joos U, Kleinheinz J.Reconstruction of the severely resorbed (ClassⅥ) Jaws: Routine or exception[J]. J Craniomaxillofac Surg, 2000, 28(1): 1-4.
[4] Jimi E, Hirata S, Osawa K, et al.The current and future therapies of bone regeneration to repair bone defects[J]. Int J Dent, 2012 2012(6): 148261.
[5] 赖红昌, 史俊宇. 上颌窦提升术[J]. 口腔疾病防治, 2017, 25(1): 8-12.
[6] Gu YX, Jy S, Zhuang LF, et al.Transalveolar sinus floor elevation using osteotomes without grafting in severely atrophic maxilla: a 5-year prospective study[J]. Clin Oral Implants Res, 2016, 27(1): 120-125.
[7] 赵继刚, 梁景章, 黄艳阳, 等. 环形骨块内嵌式窦底提升与常规上颌窦外提升术式的比较研究[J]. 口腔疾病防治, 2016, 24(10): 582-588.
[8] Cordaro L, Boghi F, Mirisola Di Torresanto V, et al. Reconstruction of the moderately atrophic edentulous maxilla with mandibular bone grafts[J]. Clin Oral Implants Res, 2013, 24(11): 1214-1221.
[9] 徐淑兰, 周磊, 黄建生, 等. 上置法植骨60例种植效果分析[J]. 广东医学, 2006, 27(11): 1720-1721.
[10] Cordaro L, Amadé DS, Cordaro M.Clinical results of alveolar ridge augmentation with mandibular block bone grafts in partially edentulous patients prior to implant placement[J]. Clin Oral Implants Res, 2002, 13(1): 103-111.
[11] 陈磊, 谢琳. 萎缩性牙槽骨实验动物模型的建立[J]. 吉林医学, 2011, 32(6): 1043-1044.
[12] 刘星纲, 邓旭亮, 邹超, 等. 多孔β-磷酸三钙/胶原支架修复兔下颌牙槽骨缺损的实验研究[J]. 北京口腔医学, 2014, 22(1): 1-4.
[13] 姚钟雄. 块状珊瑚羟基磷灰石成骨效能及重建下颌后牙区牙槽骨高度的研究[D]. 广州: 南方医科大学, 2014: 8-19.
[14] Aerssens J, Boonen S, Lowet G, et al.Interspecies differences in bone composition, density, and quality: potential implications for in vivo bone research[J]. Endocrinology, 1998, 139(2): 663-670.
[15] Jaul DH, Mcnamara JA, Carlson DS, et al.A cephalometric evaluation of edentulous Rhesus monkeys (Macaca mulatta): a long-term study[J]. J Prosthet Dent, 1980, 44(4): 453-460.
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