口腔疾病防治 ›› 2017, Vol. 25 ›› Issue (11): 718–722.doi: 10.12016/j.issn.2096-1456.2017.11.008

• 基础研究 • 上一篇    下一篇

IL-6 -572基因多态性与侵袭性牙周炎易感性的相关性研究

李璟1(), 梁菁1, 徐婷1, 谭凤清1, 刘娟2, 曾雄群2()   

  1. 1. 广州市海珠区口腔医院,广东 广州(510310)
    2. 南方医科大学口腔医院,广东 广州(510280)
  • 收稿日期:2017-09-10 修回日期:2017-10-10 出版日期:2017-11-20 发布日期:2018-09-03
  • 作者简介:

    【作者简介】 李璟,主治医师,硕士, Email:lijing19851208@126.com

  • 基金资助:
    广州市海珠区科技计划项目(2017-43);广东省自然科学基金(06022942)

Relationship between interleukin-6 -572 gene polymorphism and aggressive periodontitis in Chinese

Jing LI1(), Jing LIANG1, Ting XU1, Fengqing TAN1, Juan LIU2, Xiongqun ZENG2()   

  1. 1. Guangzhou Haizhuqu District Stomatological Hospital, Guangzhou 510310, China
    2. The Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
  • Received:2017-09-10 Revised:2017-10-10 Online:2017-11-20 Published:2018-09-03

摘要:

目的 研究白细胞介素-6(interleukin-6,IL-6)-572位点基因多态性与侵袭性牙周炎易感性的关系。方法 采用病例对照试验设计,从广东汉族人群中选择83例侵袭性牙周炎(aggressive periodontitis,AgP)患者(AgP组)及79例牙周健康者(对照组),采用聚合酶链反应—限制性内切酶片段长度多态性分析方法对IL-6 -572位点基因多态性进行检测,分析组间基因型频率及等位基因分布的差异。结果 IL-6基因启动子区-572位点G/C基因型在AgP组、对照组中的分布频率差异有统计学意义(χ2=13.710,P=0.001)。AgP组与对照组相比,G、C等位基因频率分布差异有统计学意义(χ2=13.213,P<0.001),G等位基因相对于C等位基因:OR值为2.988,95%CI:1.634 ~ 5.465。结论 IL-6 -572 G/C位点的基因多态性同中国广东汉族人群侵袭性牙周炎患病易感性可能存在相关关系,IL-6 -572 G等位基因可能是广东汉族人群AgP遗传易感性的高风险因素。

关键词: 白细胞介素-6, 慢性牙周炎, 侵袭性牙周炎, 单核苷酸多态性, 等位基因

Abstract:

Objective To investigate the association between interleukin-6 -572 gene polymorphism and aggressive periodontitis. Methods A case-control study involved 83 patients with aggressive periodontitis (AgP) and 69 health controls was held, and the genotype and allele distributions of interleukin-6 -572 gene polymorphism were analyzed by PCR-RFLP. Results There was statistically significant differences in the genotype distribution by the chi-square test in the two groups (χ2 = 13.710, P=0.001). The distribution of allele frequencies in AgP group was statistically different (χ2=13.213, P < 0.001) from the healthy control group, and the OR for the G allele is 2.988 (95%CI: 1.634-5.465) when compared with the C allele. Conclusion IL-6 -572 gene polymorphism is associated with the susceptibility to AgP in Chinese Han population of Guangdong, and the IL-6 -572 G allele might be a risk factor of the genetic susceptibility to AgP.

Key words: Interleukin-6, Chronic periodontitis, Aggressive periodontitis, Single nucleotide polymorphism, Allele

中图分类号: 

  • R781.4

表1

2组基线数据比较"

组别 n 男/女 年龄 PD(mm) CAL(mm)
AgP组 83 40/43 31.37 ± 4.17 5.23 ± 0.52 6.24 ± 1.27
对照组 69 30/39 42.82 ± 8.63 2.74 ± 0.38 0.0

图1

IL-6 -572G/C多态性检测酶切电泳图泳道1:空白对照;泳道M:Φ × 174-HaeⅢ DNA Marker;泳道2:PCR产物;泳道3:GG纯合子;泳道4~6:CC纯合子;泳道7、8:GC杂合子。"

表2

IL-6 -572位点基因型分布及等位基因频率比较"

组别 n 基因型分布 等位基因频率(%)
GC GG CC G C
AgP组 83 39(47.0) 13(15.7) 31(37.3) 66(39.8) 100(60.2)
对照组 69 25(36.2) 1(0.01) 43(62.3) 27(19.6) 111(80.4)
合计 152 64(42.1) 14(9.2) 74(48.7) 93(30.6) 211(69.4)
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